Coleoptera
Solutions, fluids etc
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A recommended method is to kill larvae in "Coccid preservative"and keep them in it for 2 to 3 days. Then they will be transferred to "Larval preservative" for final storage. With very large insects it is necessary to inject some "Pampel's fluid" into the body before placing the specimens in the preservative. This will prevent the decay of the internal organs.
The recipe for the preparation of each preservative is as follows:-
Coccid preservative
To make 100ml = 65 ml Ethanol (95%)
20 ml Glacial Acetic Acid
5 ml Glycerol
10 ml Distilled water

Larval preservative
To make 100 ml = 80 ml Ethanol (95%)
5 ml Glacial Acetic Acid
5 ml Glycerol
10 ml Distilled water

Pampel's fluid
To make 100 ml = 30 ml Ethanol (95%)
5 ml Glacial Acetic Acid
5 ml Glycerol
10 ml Formaldehyd (40%)
50 ml Distilled water

Pampel's fluid
, was suggested by Duffy: A Monograph of the immature stages of British and imported beetles - London, 1953: 308

Glacial acetic acid ------------------------ 4 parts
Distilled water --------------------------- 30 parts
Formaldehyde --------------------------- 6 parts
Ethyl alcohol (95 percent) --------------- 15 parts

This solution may be used to kill and to preserve larvae, but better use alcohol for study and dissection.

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Last modified on Friday, 28 April 2017